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KMID : 0613820080180081083
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Journal of Life Science
2008 Volume.18 No. 8 p.1083 ~ p.1089
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Analysis of the Diversity of Methicillin Resistant Staphylococcus aureus (MRSA) Strains Isolated from Diarrhea Patients in Busan Area
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Choi Seung-Hwa
Park Eun-Hee
Park Yeon-Kyoung
Kim Joung-A
Bin Jae-Hun
Park Ho-Kuk
Lee Young-Suk
Kim Nam-Ho
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Abstract
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We investigated the molecular epidemiological characteristic of methicillin resistant Staphylococcus aureus (MRSA) strains isolated from stool samples in Busan from 2004 to 2006. Among 142 isolates of S. aureus, 49 isolates (34.5%) were confirmed as MRSA. With the antimicrobial susceptibility tests, 37 isolates (75.5%) showed multiple resistance to more than 10 antibiotics, but all isolates were sensitive to vancomycin. All of MRSA had enterotoxin A in 30.6%, B 4.1%, C 8.3%, D, C/G, A 2.0% and None 51%. PFGE of SmaⅠ-digested chromosomal DNA was performed on 49 sporadic MRSA isolates. Restriction fragment patterns consisted of 8 to 14 fragments ranged in size from 48.5 to 630.5 kbp. We could divided the isolates into 7 groups (Ⅰ～Ⅶ) by analyzing PFGE patterns. Group I subdivided as 2 subgroups and 17 (34.7%) strains belong to the group Ⅰ. Dendrogram of PFGE patterns showed that MRSA strains in Busan were heterogeneous but we could find out minor homogeneity in hospital.
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KEYWORD
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Methicillin resistant Staphylococcus aureus (MRSA), antimicrobial susceptibility, mecA gene, enterotoxin, PFGE
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